应用DHPLC技术进行诊断性分析的质量保证体系(PPT 65页)

Normal ranges of the mutation standards
The maintenance procedure
Filter and flush
Pre-run maintenance
Weekly maintenance
Quarterly maintenance
DNASep® and DNASep® HT cartridge maintenance
Daily Maintenance
Weekly Maintenance
1000 Injection Maintenance
Storing the Cartridge
Installing a New Cartridge
 PCR requirements
Primer Design
Size of PCR fragment
Quantity of PCR fragment
Sample Preparation for DHPLC
The Importance of Polymerase Fidelity for Mutation Detection
Polymerase Fidelity Comparison
Maximum recommended concentrationsof acceptable PCR additives
Unacceptable PCR components.
Controls
Control samples in DHPLC
Instrument Buffers
Heteroduplex formation
Software
Results Interpretation
Assessment of chromatogram quality
Controls
Identification of sequence variants
Data checking, reporting and storage
Rett syndrome
Materials
Materials
Method
Interpretation of the results
Reporting procedures
NEGATIVE RESULT IN A  FEMALE
NEGATIVE RESULT IN A MALE
NORMAL PARENT
POSITIVE RESULT IN A FEMALE
诊断性DHPLC质量保证（DDQA）
DHPLC技术性能发挥
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